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goat antiflag  (Novus Biologicals)


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    Structured Review

    Novus Biologicals goat antiflag
    Goat Antiflag, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa/DYKDDDDK+Epitope+Tag+Antibody+-+BSA+Free/pm42873826-325-19-22
    Average 94 stars, based on 20 article reviews
    goat antiflag - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Efficient Reconstitution of Hepatic Microvasculature by Endothelin Receptor Antagonism in Liver Sinusoidal Endothelial Cells
    Article Snippet: Assays for cell attachment used 4 × 10 5 LSEC/cm 2 of collagen-coated dishes in Medium-199 (Sigma–Aldrich) with 100 μg/mL Endothelial Cell Growth Factor and 20% fetal bovine serum (M-199) for 4 h. Cells were counted by Operetta high-content imaging (PerkinElmer, New York, NY). .. For F-actin and vinculin staining, cells were plated in collagen-coated dishes for 2 h. For F-actin, cells were fixed with ethanol, permeabilized with 0.1% Triton X-100 (Sigma–Aldrich) in phosphate-buffered saline (PBS) for 10 min, blocked in 3% bovine serum albumin (BSA) for 30 min, and then incubated with phalloidin in 1% BSA (1:250, Phalloidin Cruz FluorTM 488 Conjugate; S.C-363791; Santa Cruz Biotechnology, Santa Cruz, CA) for 30 min. For vinculin, cells were incubated with anti-vinculin in 1% BSA (1:250, NB600-1293; Novus Biologicals, Littleton, CO) overnight at 4°C followed by TRITC-conjugated anti-mouse immunoglobulin G (IgG; 1:250, T5393; Sigma–Aldrich) for 1 h at room temperature. ..

    Saline:

    Article Title: Efficient Reconstitution of Hepatic Microvasculature by Endothelin Receptor Antagonism in Liver Sinusoidal Endothelial Cells
    Article Snippet: Assays for cell attachment used 4 × 10 5 LSEC/cm 2 of collagen-coated dishes in Medium-199 (Sigma–Aldrich) with 100 μg/mL Endothelial Cell Growth Factor and 20% fetal bovine serum (M-199) for 4 h. Cells were counted by Operetta high-content imaging (PerkinElmer, New York, NY). .. For F-actin and vinculin staining, cells were plated in collagen-coated dishes for 2 h. For F-actin, cells were fixed with ethanol, permeabilized with 0.1% Triton X-100 (Sigma–Aldrich) in phosphate-buffered saline (PBS) for 10 min, blocked in 3% bovine serum albumin (BSA) for 30 min, and then incubated with phalloidin in 1% BSA (1:250, Phalloidin Cruz FluorTM 488 Conjugate; S.C-363791; Santa Cruz Biotechnology, Santa Cruz, CA) for 30 min. For vinculin, cells were incubated with anti-vinculin in 1% BSA (1:250, NB600-1293; Novus Biologicals, Littleton, CO) overnight at 4°C followed by TRITC-conjugated anti-mouse immunoglobulin G (IgG; 1:250, T5393; Sigma–Aldrich) for 1 h at room temperature. ..

    Article Title: Generation of Rat Monoclonal Antibody for Cytokeratin 18 by Immunization of Three-Dimensional-Cultured Cancer Cells.
    Article Snippet: Cytokeratin (CK) 18 is an intermediate filament protein that plays a major functional role in the integrity and mechanical stability of cells.. Since both CK8 and CK18 are major components of simple epithelia, in the context of tumors, they are expressed in most carcinomas, and have been studied as diagnostic and prognostic markers in tumor pathology.. CK18 is also cleaved by some caspases during apoptosis.

    Incubation:

    Article Title: Efficient Reconstitution of Hepatic Microvasculature by Endothelin Receptor Antagonism in Liver Sinusoidal Endothelial Cells
    Article Snippet: Assays for cell attachment used 4 × 10 5 LSEC/cm 2 of collagen-coated dishes in Medium-199 (Sigma–Aldrich) with 100 μg/mL Endothelial Cell Growth Factor and 20% fetal bovine serum (M-199) for 4 h. Cells were counted by Operetta high-content imaging (PerkinElmer, New York, NY). .. For F-actin and vinculin staining, cells were plated in collagen-coated dishes for 2 h. For F-actin, cells were fixed with ethanol, permeabilized with 0.1% Triton X-100 (Sigma–Aldrich) in phosphate-buffered saline (PBS) for 10 min, blocked in 3% bovine serum albumin (BSA) for 30 min, and then incubated with phalloidin in 1% BSA (1:250, Phalloidin Cruz FluorTM 488 Conjugate; S.C-363791; Santa Cruz Biotechnology, Santa Cruz, CA) for 30 min. For vinculin, cells were incubated with anti-vinculin in 1% BSA (1:250, NB600-1293; Novus Biologicals, Littleton, CO) overnight at 4°C followed by TRITC-conjugated anti-mouse immunoglobulin G (IgG; 1:250, T5393; Sigma–Aldrich) for 1 h at room temperature. ..

    Article Title: Loss of Function of WFS1 Causes ER Stress-Mediated Inflammation in Pancreatic Beta-Cells.
    Article Snippet: For the DAB stained samples, endogenous peroxidase was quenched using BLOXALL Blocking solution (VECTOR, Cat# SP-6000). .. Blocking was performed in 2% bovine serum albumin (BSA) for 1 h. The following primary antibodies were diluted in 0.2% BSA and incubated overnight at 4°C: anti-WFS1 (Proteintech, Cat# 1158-1-AP, 1:100), anti-Iba1 (Novus, Cat# NB100-1028, 1:50), anti-CD31 (abcam, Cat# ab124432, 1:100), and Alexa-Fluor 488 conjugated anti-insulin (Invitrogen, Cat# 53-9769-82, 1:100). .. After washing the primary antibodies 3 times with PBS, the tissues were incubated for 30 min at room temperature with ImmPRESS polymer reagent (VECTOR, Cat# MP-7401-50) for DAB staining, or AlexaFluor 594 donkey anti-rabbit IgG (Invitrogen, Cat# A21207) for immunofluorescent staining, and washed for 2 times with PBS.

    Article Title: Transcriptomic profiling of trophoblast fusion using BeWo and JEG-3 cell lines.
    Article Snippet: In human placenta, alteration in trophoblast differentiation has a major impact on placental maintenance and integrity.. However, little is known about the mechanisms that control cytotrophoblast fusion.. The BeWo cell line is used to study placental function, since it forms syncytium and secretes hormones after treatment with cAMP or forskolin.

    Article Title: Precision mRNA Nanomedicine for Targeted Vascular Therapies in ARDS and Atherosclerosis
    Article Snippet: .. After permeabilization with 0.3% Triton X-100 for 30 minutes and blocking with 2% BSA for 1 hour at room temperature, the arteries were incubated overnight at 4°C with primary antibodies (Anti-Flag, #NBP1-06712, Novus; VE-Cadherin, #ab33168, Abcam). .. Following PBS washes, arteries were incubated with Alexa Fluor 594-labeled anti-rabbit and Alexa Fluor 647-labeled anti-rat secondary antibodies (Invitrogen) for 1 hour at room temperature.

    Article Title: XRCC1 mediates PARP1- and PAR-dependent recruitment of PARP2 to DNA damage sites.
    Article Snippet: .. Fixed cells were permeabilized with 0.2% Triton X-100 in PBS for 10 min and then blocked with 3% BSA in PBS at room temperature for 1 h. Cells were incubated with primary antibodies diluted in 3% BSA (anti-XRCC1, Novus Biologicals, Cat. NBP1-87154, 1:500) overnight at 4 ◦C, then three 5- min washes with PBS. ..

    Article Title: Generation of Rat Monoclonal Antibody for Cytokeratin 18 by Immunization of Three-Dimensional-Cultured Cancer Cells.
    Article Snippet: Cytokeratin (CK) 18 is an intermediate filament protein that plays a major functional role in the integrity and mechanical stability of cells.. Since both CK8 and CK18 are major components of simple epithelia, in the context of tumors, they are expressed in most carcinomas, and have been studied as diagnostic and prognostic markers in tumor pathology.. CK18 is also cleaved by some caspases during apoptosis.

    Blocking Assay:

    Article Title: Loss of Function of WFS1 Causes ER Stress-Mediated Inflammation in Pancreatic Beta-Cells.
    Article Snippet: For the DAB stained samples, endogenous peroxidase was quenched using BLOXALL Blocking solution (VECTOR, Cat# SP-6000). .. Blocking was performed in 2% bovine serum albumin (BSA) for 1 h. The following primary antibodies were diluted in 0.2% BSA and incubated overnight at 4°C: anti-WFS1 (Proteintech, Cat# 1158-1-AP, 1:100), anti-Iba1 (Novus, Cat# NB100-1028, 1:50), anti-CD31 (abcam, Cat# ab124432, 1:100), and Alexa-Fluor 488 conjugated anti-insulin (Invitrogen, Cat# 53-9769-82, 1:100). .. After washing the primary antibodies 3 times with PBS, the tissues were incubated for 30 min at room temperature with ImmPRESS polymer reagent (VECTOR, Cat# MP-7401-50) for DAB staining, or AlexaFluor 594 donkey anti-rabbit IgG (Invitrogen, Cat# A21207) for immunofluorescent staining, and washed for 2 times with PBS.

    Article Title: Precision mRNA Nanomedicine for Targeted Vascular Therapies in ARDS and Atherosclerosis
    Article Snippet: .. After permeabilization with 0.3% Triton X-100 for 30 minutes and blocking with 2% BSA for 1 hour at room temperature, the arteries were incubated overnight at 4°C with primary antibodies (Anti-Flag, #NBP1-06712, Novus; VE-Cadherin, #ab33168, Abcam). .. Following PBS washes, arteries were incubated with Alexa Fluor 594-labeled anti-rabbit and Alexa Fluor 647-labeled anti-rat secondary antibodies (Invitrogen) for 1 hour at room temperature.

    Article Title: Generation of Rat Monoclonal Antibody for Cytokeratin 18 by Immunization of Three-Dimensional-Cultured Cancer Cells.
    Article Snippet: Cytokeratin (CK) 18 is an intermediate filament protein that plays a major functional role in the integrity and mechanical stability of cells.. Since both CK8 and CK18 are major components of simple epithelia, in the context of tumors, they are expressed in most carcinomas, and have been studied as diagnostic and prognostic markers in tumor pathology.. CK18 is also cleaved by some caspases during apoptosis.

    other:

    Article Title: Macrophages promote anti-androgen resistance in prostate cancer bone disease
    Article Snippet: Images were taken using confocal Zeiss LSM 710 Microscope.

    Article Title: XRCC1 mediates PARP1- and PAR-dependent recruitment of PARP2 to DNA damage sites
    Article Snippet: Transfected cells were washed with cold PBS and lysed in 200 μl of NP-40 lysis buffer (25 mM Tris–HCl, pH 7.4, 150 mM NaCl, 1% NP-40, 10% glycerol, and 2 mM MgCl 2 ) with protease inhibitor cocktail, phosphatase inhibitors, 1 μM olaparib, 1 μM PARG inhibitor PDD00017273 and 0.2% Benzonase (Millipore, 71205-M) at 4°C.

    Cell Culture:

    Article Title: Generation of Rat Monoclonal Antibody for Cytokeratin 18 by Immunization of Three-Dimensional-Cultured Cancer Cells.
    Article Snippet: Cytokeratin (CK) 18 is an intermediate filament protein that plays a major functional role in the integrity and mechanical stability of cells.. Since both CK8 and CK18 are major components of simple epithelia, in the context of tumors, they are expressed in most carcinomas, and have been studied as diagnostic and prognostic markers in tumor pathology.. CK18 is also cleaved by some caspases during apoptosis.



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